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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Pineal Res. 2018 Jun 6;65(3):e12509. doi: 10.1111/jpi.12509

Figure 4.

Figure 4

Rhythmicity of melatonin biosynthesis and melatonin-dependent processes was restored in congenic animals. A: Subjective day/subjective night difference of pineal gland melatonin content in the N3 animals. Pineal glands were collected at CT6 (subjective day) and CT20 or CT21-22 (subjective night) and melatonin assayed by HPLC. Each dot represents an individual animal; group means are represented by short lines. *** P = 8.40 × 10−5, ** P = 0.004 (day vs CT21-22) or P = 0.008 (CT20 vs CT21-22), 2-tailed unpaired Student’s t-test. B: Subjective day/subjective night difference of retinal dopamine release in CBA, B6 and B6 congenic animals. Eyeballs were collected at CT6 (subjective day) and CT18-20 (subjective night) in the dark. Contents of DA and DOPAC were measured with HPLC-ECD. DA release was represented by DOPAC/DA ratio. Data are mean ± S.E.M. * P = 0.0175, *** P = 6.95 × 10−5, n.s. not significant, 2-tailed unpaired Student’s t-test. n = 4 for CBA and B6, at each time point and 6 for B6 congenic animals, at each time point.