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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Hippocampus. 2018 Aug;28(8):586–601. doi: 10.1002/hipo.22962

Figure 2. Marker expression and morphology of neurons and astrocytes in the adult Nestin lineage.

Figure 2

(A) Representative images of immature neurons co-expressing EYFP and doublecortin (DCX). (B) Representative images of a mature neuron co-expressing EYFP and NeuN. (C) Representative images of a radial glial-like cell (RGL) co-expressing EYFP, GFAP, and Nestin with cell body in the subgranular zone (SGZ) and radial process traversing the granule cell layer. (D) Representative images of stellate astrocytes (SA) co-expressing EYFP and GFAP with multiple similar-sized processes extending from the cell body. Arrowhead points to S100β+ SA. Arrows point to S100β- SAs. (E) Representative images of atypical astrocytes (AA) co-expressing EYFP and GFAP with a single dominant process extending from the cell body, which are in the outer GCL. Arrow points to Nestin+ AA with one process. Arrowhead points to S100β+ AA with one dominant process and multiple minor processes. Scale bar represents 10 μM. Dotted lines indicate SGZ.