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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Exp Eye Res. 2018 Mar 31;178:228–237. doi: 10.1016/j.exer.2018.03.022

Fig 4. Assessment of Nrf2 gene and protein in Müller cells exposed to Hhcy.

Fig 4

Relative Nrf2 mRNA expression levels in Müller cells (A) exposed to 50μM and 1mM Hcy for 3h and 8h; (B) Müller cells exposed to 50 μM Hcy for 1h, 3h, 6h, 8h and 18h, n=3, *p<0.05, **p<0.01 (Kruskal-Wallis and Dunn’s multiple comparison tests). (C) Western blot analysis of NRF2 protein levels in Müller cells exposed to 50μM Hcy for 3h and 20h, TBHQ is a positive inducer of Nrf2. (D) Quantification of data shown in panel F (ns = not significant, Krusal-Wallis and Dunn’s). (E) Detection of NRF2 in the nuclear and cytoplasmic fractions of Müller cells exposed 4h to 50 μM or 1 mM Hcy; HDAC1 was used as a marker for the nuclear fraction and GAPDH for the cytosolic fraction.