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. 2018 Oct;84:98–102. doi: 10.1016/j.cryobiol.2018.07.008

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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Flow cytometry plots of cryopreserved and thawed limbal suspension cultures showing the presence or absence of a Side Population fraction which extrude Hoechst. (A) uncryopreserved, cultured human fibroblasts in the presence of Hoechst. The trapezoid encapsulates the SP fraction (P2). (B) uncryopreserved human limbal epithelial cells in the presence of Hoechst. The trapezoid encapsulates the SP fraction (P2 – shown in green). (C) cryopreserved cultured human limbal epithelial cells in the presence of Hoechst. The trapezoid encapsulates the SP fraction (P2 – shown in green). The SP fraction in cryopreserved cultured human limbal epithelial cells is obliterated by incubation with Verapamil (D) and Fumitremorgin (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)