REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal antibody PY72	InVivo Biotech Services	P172.1
Rabbit anti EGFR pY1045	Cell Signaling Technology	Cat. # 2237
Rabbit anti EGFR pY1068	Cell Signaling Technology	Cat. # 3777
Mouse anti EGFR pY845	BD Biosciences	Cat. # 558381
Goat anti EGFR	R&D Systems	Cat. # AF231
Alexa Fluor® 568 donkey anti-rabbit IgG	Life Technologies	Cat. # A10042
Alexa Fluor® 568 donkey anti-mouse IgG	Life Technologies	Cat. # A10037
Alexa Fluor® 647 donkey anti-goat IgG	Life Technologies	Cat. # A-21447
Alexa Fluor® 647 chicken anti-mouse IgG	Life Technologies	Cat. # A-21463
Alexa Fluor® 647 donkey anti-rabbit IgG	Life Technologies	Cat. # A-31573
IRDye® 800CW Donkey anti-Rabbit IgG	Licor	Cat. # 926-32213
IRDye® 680RD Donkey anti-Mouse IgG	Licor	Cat. # 926-68072
Goat anti GFP	Abcam	Cat. #ab6673
Anti-GFP antibody	Clontech	Cat. #632375
Anti-Sulfenic acid modified cysteine antibody	Millipore	Cat. #ABS30
Anti-EGFR antibody	Cell Signaling Technology	Cat. #4267
Bacterial and Virus Strains
E. coli (XL10 - Gold ultracompetent) cells	Stratagene	Cat. # 200314
Chemicals, Peptides, and Recombinant Proteins
Dulbecco’s modified Eagle’s medium (DMEM)	PAN Biotech	Cat. # P04-01500
Dulbecco’s modified Eagle’s medium (DMEM / F12)	PAN Biotech	Cat. # P04-41450
MEM Amino Acids Solution (50x)	PAN Biotech	Cat. # P08 32100
Penicillin- Streptomycin	PAN Biotech	Cat. # P06 07100
Fetal Bovine Serum	Sigma-Aldrich	Cat. # F7524
EGF	Sigma-Aldrich	Cat. # E9644
Hydrocortisone	Sigma-Aldrich	Cat. #H-0888
Cholera toxin	Sigma-Aldrich	Cat. #C-8052
Insulin	Sigma-Aldrich	Cat. #I-1882
Cy3.5® NHS ester	GE Healthcare	Cat. # PA23501
FuGENE6	Roche Diagnostics	Cat. # 11 815 091
Roti® Histofix	Carl Roth	Cat. # P087
Odyssey Blocking buffer	LI-COR	Cat. # 927
RNA Later	Thermo Fisher Scientific	Cat. # AM7020
PF6-AM	Dickinson et al., 2011	Prof Christopher J. Chang, University of California, Berkeley
N-Ethylmaleimide	Sigma-Aldrich	Cat. # 128-53-0
Dimedone	Sigma-Aldrich	Cat. # 38490
Phusion Flash High-Fidelity PCR Master Mix	Thermo Fisher Scientific	Cat. # F548S
Herculase II Fusion DNA Polymerase	Agilent	Cat. # 600675
Triton-X100	Serva	Cat. # 37240
Alexa647-Malemide	Thermo Fisher Scientific	Cat. # A20347
N,N dimethylformamide	Acros Organics	Cat. # 348435000
Bicine	Sigma-Aldrich	Cat. # B3876
Diphenyleneiodonium	Sigma-Aldrich	Cat. # D2926
Critical Commercial Assays
MycoAlert Mycoplasma detection kit	Lonza	Cat. # LT07-218
RNeasy Maxi Kit	QIAGEN	Cat. # 75162
Oligotex mRNA Midi Kit	QIAGEN	Cat. # 70042
AffinityScript Multiple Temperature cDNA Synthesis Kit	Agilent	Cat. #200436
BigDye® Terminator v3.1 Cycle Sequencing Kit	Thermo Fisher Scientific	Cat. # 4337455
PureYield plasmid Midiprep System	Promega	Cat. # A2492
NucleoBond® Xtra Midi EF	Macherey-Nagel	Cat. # 740420.10
Experimental Models: Cell Lines
MCF-7	ECACC	Cat. No. 86012803
MCF7-EG	Roda-Navaro and Bastiaens, 2014	N/A
MCF10A	ATCC	CRL - 10317
Oligonucleotides
PTPX and CYBA siRNA SMARTpool	Dharmacon	Additional Data Table S3 (separate file)
Recombinant DNA
cHyPer3	Bilan et al., 2013	Prof. Vsevolod Belousov, Shemyakin–Ovchinnikov Institute of bioorganic chemistry, Moscow
His-CBD-Intein-(Cys)-hEGF-(Cys)	Sonntag et al., 2014	Prof. Luc Brunsveld, University of Technology, Eindhoven
cDNA-mCitrine expression plasmid constructs	This paper	Additional Data Table S2 (separate file)
EGFR-mCitrine-N1	Baumdick et al., 2015	N/A
PTB-mCherry	Fueller et al., 2015	N/A
cCbl-BFP	Fueller et al., 2015	N/A
Rab11-S25N-BFP	Konitsiotis et al., 2017	N/A
Software and Algorithms
CellProfiler	Kamentsky et al., 2011	http://cellprofiler.org
R Statistical Software	Foundation for Statistical Computing	https://www.r-project.org/
XPPAUT		www.math.pitt.edu/∼bard/xpp/xpp.html)
MATLAB	MathWorks	http://mathworks.com/
Python	Python Software Foundation	https://www.python.org/
Scikit-image	Version 0.11.3	www.scikit-image.org
OpenCV	Version 2.4.8	http://opencv.org
ImageJ/Fiji		https://fiji.sc/
IgorPro 6.37	Wavemetrics	www.igorpro.net
Other
7K Zeba Spin Desalting Columns	Thermo Scientific	Cat. # 89882
Dynabeads® Protein G magnetic beads	Thermo Fisher Scientific	Cat. # 10003D
NuPAGE Novex 4-12% Bis-Tris gels	ThermoFisher	Cat. # NP0335BOX
Cellasic ONIX plates	Merck Chemicals	M04S