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. 2018 Sep 26;7(3):295–309.e11. doi: 10.1016/j.cels.2018.06.006
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Mouse monoclonal antibody PY72 InVivo Biotech Services P172.1
Rabbit anti EGFR pY1045 Cell Signaling Technology Cat. # 2237
Rabbit anti EGFR pY1068 Cell Signaling Technology Cat. # 3777
Mouse anti EGFR pY845 BD Biosciences Cat. # 558381
Goat anti EGFR R&D Systems Cat. # AF231
Alexa Fluor® 568 donkey anti-rabbit IgG Life Technologies Cat. # A10042
Alexa Fluor® 568 donkey anti-mouse IgG Life Technologies Cat. # A10037
Alexa Fluor® 647 donkey anti-goat IgG Life Technologies Cat. # A-21447
Alexa Fluor® 647 chicken anti-mouse IgG Life Technologies Cat. # A-21463
Alexa Fluor® 647 donkey anti-rabbit IgG Life Technologies Cat. # A-31573
IRDye® 800CW Donkey anti-Rabbit IgG Licor Cat. # 926-32213
IRDye® 680RD Donkey anti-Mouse IgG Licor Cat. # 926-68072
Goat anti GFP Abcam Cat. #ab6673
Anti-GFP antibody Clontech Cat. #632375
Anti-Sulfenic acid modified cysteine antibody Millipore Cat. #ABS30
Anti-EGFR antibody Cell Signaling Technology Cat. #4267

Bacterial and Virus Strains

E. coli (XL10 - Gold ultracompetent) cells Stratagene Cat. # 200314

Chemicals, Peptides, and Recombinant Proteins

Dulbecco’s modified Eagle’s medium (DMEM) PAN Biotech Cat. # P04-01500
Dulbecco’s modified Eagle’s medium (DMEM / F12) PAN Biotech Cat. # P04-41450
MEM Amino Acids Solution (50x) PAN Biotech Cat. # P08 32100
Penicillin- Streptomycin PAN Biotech Cat. # P06 07100
Fetal Bovine Serum Sigma-Aldrich Cat. # F7524
EGF Sigma-Aldrich Cat. # E9644
Hydrocortisone Sigma-Aldrich Cat. #H-0888
Cholera toxin Sigma-Aldrich Cat. #C-8052
Insulin Sigma-Aldrich Cat. #I-1882
Cy3.5® NHS ester GE Healthcare Cat. # PA23501
FuGENE6 Roche Diagnostics Cat. # 11 815 091
Roti® Histofix Carl Roth Cat. # P087
Odyssey Blocking buffer LI-COR Cat. # 927
RNA Later Thermo Fisher Scientific Cat. # AM7020
PF6-AM Dickinson et al., 2011 Prof Christopher J. Chang, University of California, Berkeley
N-Ethylmaleimide Sigma-Aldrich Cat. # 128-53-0
Dimedone Sigma-Aldrich Cat. # 38490
Phusion Flash High-Fidelity PCR Master Mix Thermo Fisher Scientific Cat. # F548S
Herculase II Fusion DNA Polymerase Agilent Cat. # 600675
Triton-X100 Serva Cat. # 37240
Alexa647-Malemide Thermo Fisher Scientific Cat. # A20347
N,N dimethylformamide Acros Organics Cat. # 348435000
Bicine Sigma-Aldrich Cat. # B3876
Diphenyleneiodonium Sigma-Aldrich Cat. # D2926

Critical Commercial Assays

MycoAlert Mycoplasma detection kit Lonza Cat. # LT07-218
RNeasy Maxi Kit QIAGEN Cat. # 75162
Oligotex mRNA Midi Kit QIAGEN Cat. # 70042
AffinityScript Multiple Temperature cDNA Synthesis Kit Agilent Cat. #200436
BigDye® Terminator v3.1 Cycle Sequencing Kit Thermo Fisher Scientific Cat. # 4337455
PureYield plasmid Midiprep System Promega Cat. # A2492
NucleoBond® Xtra Midi EF Macherey-Nagel Cat. # 740420.10

Experimental Models: Cell Lines

MCF-7 ECACC Cat. No. 86012803
MCF7-EG Roda-Navaro and Bastiaens, 2014 N/A
MCF10A ATCC CRL - 10317

Oligonucleotides

PTPX and CYBA siRNA SMARTpool Dharmacon Additional Data Table S3 (separate file)

Recombinant DNA

cHyPer3 Bilan et al., 2013 Prof. Vsevolod Belousov, Shemyakin–Ovchinnikov Institute of bioorganic chemistry, Moscow
His-CBD-Intein-(Cys)-hEGF-(Cys) Sonntag et al., 2014 Prof. Luc Brunsveld, University of Technology, Eindhoven
cDNA-mCitrine expression plasmid constructs This paper Additional Data Table S2 (separate file)
EGFR-mCitrine-N1 Baumdick et al., 2015 N/A
PTB-mCherry Fueller et al., 2015 N/A
cCbl-BFP Fueller et al., 2015 N/A
Rab11-S25N-BFP Konitsiotis et al., 2017 N/A

Software and Algorithms

CellProfiler Kamentsky et al., 2011 http://cellprofiler.org
R Statistical Software Foundation for Statistical Computing https://www.r-project.org/
XPPAUT www.math.pitt.edu/∼bard/xpp/xpp.html)
MATLAB MathWorks http://mathworks.com/
Python Python Software Foundation https://www.python.org/
Scikit-image Version 0.11.3 www.scikit-image.org
OpenCV Version 2.4.8 http://opencv.org
ImageJ/Fiji https://fiji.sc/
IgorPro 6.37 Wavemetrics www.igorpro.net

Other

7K Zeba Spin Desalting Columns Thermo Scientific Cat. # 89882
Dynabeads® Protein G magnetic beads Thermo Fisher Scientific Cat. # 10003D
NuPAGE Novex 4-12% Bis-Tris gels ThermoFisher Cat. # NP0335BOX
Cellasic ONIX plates Merck Chemicals M04S