Table 2.
Multiple reaction monitoring (MRM) parameters of GAA, HA and IAA.
| Parameters | GAA | HA | IAA | SB |
|---|---|---|---|---|
| Precursor Ion (m/z) | 118.1 | 180.1 | 176.1 | 232 |
| Fragmentor (V) | 90 | 60 | 91 | 90 |
| Quantifier transition production (m/z) with CE (V) | 76 (12) |
105 (12) |
130.1 (16) |
187.9 (10) |
| Qualifier transition production (m/z) with CE (V) | 101 (8) |
77 (36) |
103.1 (36) |
140 (10) |
| CAV (V) | 4 | 4 | 4 | 4 |
| Dwell time (ms) | 30 | 30 | 30 | 30 |
| Polarity | (+) | (+) | (+) | (−) |
Guanidinoacetic acid (GAA); hippuric acid (HA); indoleacetic acid (IAA); sulbactam (SB); cell accelerator voltage (CAV); collision energy (CE).
For mass spectrometry, dry gas temperature, dry gas flow, nebulizer, sheath gas temperature, sheath gas flow, capillary voltage, EMV voltage and nozzle voltage were set at 300 °C, 5 L/min, 35 psi, 250 °C, 11 L/min, 4000 V for positive and −3500 V for negative mode, 250 V (both positive and negative mode) and 500 V, respectively.