Fig. 1.
Overall structure of MdfA in the outward open (Oo) and inward facing (If) states. a The transporter in the Oo conformation (this work); b MdfA in the ligand-bound If state (ref. 14). The N- (white/gray) and C-terminal (yellow) six transmembrane helical domains are shown in ribbon representation, with transmembrane helices (TMs) numbered. Note the difference in relative orientation of the two domains by 33.5°. TM5, whose conformation differs between the two states, is shown in green (Oo) or orange (If); the TM1–TM2 termini are in corresponding light colors. The position of chloramphenicol bound in the If state is depicted using blue sticks