Fig. 2. FIBP knockdown inhibited proliferation of CRC cells in vitro and in vivo.

a The expression of FIBP in various CRC cell lines was quantitated by qPCR. HCT116-CSC is the CSC-enriched chemoresistant cell line derived from parental HCT116 cells. n = 8 for each tested cell line. b Proliferation curves of CRC SW620, SW480, and HCT116 and HCT116-CSC cell lines with or without FIBP knockdown. c Colony-formation assays of SW620 cells and HCT116-CSCs with control or FIBP-shRNA lentivirus infection. d–g Nude mice were inoculated with SW620 cells or HCT116-CSCs pre-infected with control or FIBP-shRNA lentivirus and were sacrificed 5 weeks later. Images (d) and weight (e) of CRC xenograft tumors are shown. f Growth of xenograft tumors derived from SW620 cells and HCT116-CSCs with or without FIBP knockdown. g Respective immunochemistry staining of FIBP and Ki67 expression in tumors with or without FIBP knockdown (magnification, ×200). *p < 0.05, compared with the Vector control group