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. 2018 Sep 25;9:1018. doi: 10.3389/fphar.2018.01018

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Copyright © 2018 Tee, Peng, Tan, Yu and Ho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MgIG increased p27 expression and β-galactosidase activity in TGF-β-activated LX2 cells. (A) Western blot and densitometric analysis (against GAPDH loading control) of the proteins showed that MgIG induced expression of p27 senescence marker particularly at 24 h treatment. Cells treated with 5 μM camptothecin for 24 and 48 h, respectively, were used as positive controls. (B) TGF-β-activated LX2 cells treated with three different concentrations of MgIG for 48 h exhibited an increase in β-galactosidase activity. Data represents means ± SE, n = 3, one-way ANOVA with Tukey HSD test, p-value < 0.05, ^∗significance against non-treated control (NegCtrl) at 48 h.