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. 2018 Sep 25;9:2173. doi: 10.3389/fimmu.2018.02173

Figure 2.

Figure 2

Hyperoxaluria-induced nephrocalcinosis is associated with tubular PTX3 secretion. Female PTX3-competent (Ptx3+/+) and -deficient (Ptx3−/−) animals with 10–12 weeks of age received either control or oxalate chow for 3 weeks (n = 5). (A) Tubular expression of PTX3 was analyzed by immunohistochemical staining in frozen kidney sections and quantified as positive stained area per section in percent. (B) Detection of PTX3 in whole urine samples by immunoblotting (25 μg protein/lane, upper right). Ponceau Red staining shows major urinary proteins (mainly a2u-globulins, ~20 kDa, lower right) as a loading control. Optical density of the immunoreactive and Ponceau Red stained bands was quantified by ImageJ (NIH, Bethesda, MD). Data are from two independent experiments. n.s., not significant; *p < 0.05, ***p < 0.001 between groups as indicated.