Skip to main content
. 2018 Sep 25;9:2173. doi: 10.3389/fimmu.2018.02173

Figure 4.

Figure 4

Lack of PTX3 induces hyperoxaluria-induced progressive CKD in non-susceptible B6;129 mice. Ptx3+/+ and Ptx3−/− female littermates (10–12 weeks of age) were fed with oxalate chow for 3 weeks (n = 9). (A) Quantification of tubular injury was conducted using PAS-stained kidney sections as shown in (B). (C) Phase contrast image of crystal plugs in H&E-stained kidney section in 200× (left) and 400× (right) magnification. (D) Quantification of αSMA-positive area in kidney sections after 3 weeks of either control or oxalate chow feeding as shown in (E). (F) Percentage of CD45+ cells within whole kidney cell suspension assessed by flow cytometry. (G) GFR assessed by transcutaneous measurement of FITC-sinistrin from subcutaneous capillaries at weeks 0, 1, 2, and 3 after onset of high oxalate diet in both genotypes. Data are from four independent experiments and represent means ± SEM in (G). n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001 vs. wild type and respective #, ##, and ### vs. week 1 as indicated.