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. 2018 Sep 25;9:2119. doi: 10.3389/fimmu.2018.02119

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Copyright © 2018 Schirmer, Bringmann, Seifert and Neumann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Th1/Th2-type transcription factor expression in cola. IL-5-deficient mice were either left not manipulated (none) or were reconstituted with eosinophils obtained from IL-5-competent isogeneic wild-type (H₄R^+/+) or H₄R-deficient (H₄R^−/−) mice and then fed with water either or not containing DSS for 7 days. At day seven, the mice were dissected and cola were obtained. RNA was extracted from cola and the relative quantity of T-bet (A) and GATA3 (B) was analyzed by RT-qPCR (single values and means ± 95% CI; 1-way ANOVA with Holm-Sidak's post-test: *p < 0.05, ***p < 0.001).