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. 2018 Sep 25;9:2157. doi: 10.3389/fimmu.2018.02157

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Copyright © 2018 Bonacini, Soriano, Zerbini, Calò, Cimino, Muratore, Fontana, Braglia, Parmeggiani, Salvarani and Croci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NKG2D expression in lymphocytes from BD patients and HC. (A) Dot plot visualization of the percentages of NKG2D^pos cells in the NK, NKT, and T lymphocyte gate determined by flow cytometry in PBMCs from BD patients () and HC (■). Horizontal lines show the median ± IQR. Data were analyzed by Mann-Whitney U test. (B) ROC curve analysis of the percentages of NKG2D^pos cells in NK, NKT, and T lymphocyte gates. AUC, Area Under the Curve; CI, Confidence Interval; Thr, Threshold; Se, Sensitivity; Sp, Specificity. (C) Dot plot visualization of the correlation between the frequencies of NKG2D^pos NK, NKT, and T cells and BDCAF scores for each BD patient. Data were analyzed by Spearman's correlation test (n = 38). (D) Box plot visualization of the frequencies of NKG2D^pos NK, NKT, and T cells in BD patients with BDCAF = 0 vs. BDCAF ≥ 3 and HC. Data were analyzed by Mann-Whitney U test.

Inline graphic — NKG2D expression in lymphocytes from BD patients and HC. (A) Dot plot visualization of the percentages of NKG2D^pos cells in the NK, NKT, and T lymphocyte gate determined by flow cytometry in PBMCs from BD patients () and HC (■). Horizontal lines show the median ± IQR. Data were analyzed by Mann-Whitney U test. (B) ROC curve analysis of the percentages of NKG2D^pos cells in NK, NKT, and T lymphocyte gates. AUC, Area Under the Curve; CI, Confidence Interval; Thr, Threshold; Se, Sensitivity; Sp, Specificity. (C) Dot plot visualization of the correlation between the frequencies of NKG2D^pos NK, NKT, and T cells and BDCAF scores for each BD patient. Data were analyzed by Spearman's correlation test (n = 38). (D) Box plot visualization of the frequencies of NKG2D^pos NK, NKT, and T cells in BD patients with BDCAF = 0 vs. BDCAF ≥ 3 and HC. Data were analyzed by Mann-Whitney U test.