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. 2018 Sep 25;9:2157. doi: 10.3389/fimmu.2018.02157

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Copyright © 2018 Bonacini, Soriano, Zerbini, Calò, Cimino, Muratore, Fontana, Braglia, Parmeggiani, Salvarani and Croci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD107a degranulation assay of PBMCs from BD patients and HC. Dot plot visualization of the percentages of CD107a^pos cells in the NK lymphocyte gate determined by flow cytometry in PMBCs from BD patients () and HC (■) after stimulation with K562 in presence of 10 μg/mL brefeldin A and 6 μg/mL monensin. The assay was conducted without (A) or with 1ng/mL IL-15 (B) to maximize the stimulus. Horizontal lines show the median ± IQR. Data were analyzed by Mann-Whitney U test.

Inline graphic — CD107a degranulation assay of PBMCs from BD patients and HC. Dot plot visualization of the percentages of CD107a^pos cells in the NK lymphocyte gate determined by flow cytometry in PMBCs from BD patients () and HC (■) after stimulation with K562 in presence of 10 μg/mL brefeldin A and 6 μg/mL monensin. The assay was conducted without (A) or with 1ng/mL IL-15 (B) to maximize the stimulus. Horizontal lines show the median ± IQR. Data were analyzed by Mann-Whitney U test.