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. 2018 Sep 25;11:337. doi: 10.3389/fnmol.2018.00337

Figure 1.

Figure 1

Effect of dihydrotestosterone (DHT) on SHANK gene expression in SH-SY5Y cells. (A) Quantification of SHANK, MECP2 and PSD95 gene expression by qPCR after 4 h of treatment with either 100 nM DHT or a combination of 100 nM DHT and 1 μM flutamide (n = 5 experiments with six biological replicates for each condition). Gene expression was normalized against five reference genes (18S, GAPDH, HPRT1, HSPD1 and SDHA; two-way ANOVA, ***P ≤ 0.001, vs. mock). Bonferroni threshold: n = 5 tests, P ≤ 0.01. (B) An increase of SHANK protein levels by 50% was identified after 48 h of 100 nM DHT treatment by western blot analysis, whereas no difference was determined after 48 h of treatment with DHT combined with flutamide (n = 5 experiments, unpaired two-tailed Student’s t-tests, *P ≤ 0.05). Error bars indicate standard error of the mean (SEM).