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. 2018 Sep 25;11:337. doi: 10.3389/fnmol.2018.00337

Figure 2.

Figure 2

Effect of 17β-estradiol on SHANK gene expression in SH-SY5Y cells. Gene expression analysis of SHANK, MECP2 and PSD95 after 4 h of treatment with 100 nM 17β-estradiol. The expression was also analyzed after stimulation with 100 nM 17β-estradiol in combination with a selective blocking of estrogen receptor α (100 nM MPP), estrogen receptor β (100 nM PHTPP) or a combination of both (n = 5 experiments with six biological replicates for each condition). Gene expression was normalized against three reference genes (18S, HSPD1 and SDHA). Error bars indicate SEM (two-way ANOVA, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, vs. mock-treatment control). Bonferroni correction: n = 5 tests, P ≤ 0.01.