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. 2018 Sep 25;12:321. doi: 10.3389/fncel.2018.00321

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Copyright © 2018 Re, Dogan, Ben-Shachar, Berger, Werling, Walitza and Grünblatt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reprogramming of follicular keratinocytes. (A) Plucked human hair follicle from the scalp region. The hair follicle is in the anagen phase. A visible outer root sheath (black arrow) is highly visible and intact, thus providing a higher number of keratinocytes. (B) Confluent keratinocyte culture isolated from hair follicles, at 12 days of culture. The cells display typical keratinocyte morphology (strongly adherent cells with cobblestone appearance). (C) Representative Sendai viral generated iPSC colonies. Passage 1 iPSC culture manually picked from emerging colony in Cytotune 2.0 transfected keratinocytes. The well-defined borders of the colony are, characteristic of iPSC colonies. (D) The generated iPSC culture stains positively for TRA-1-60 (green), a known pluripotency marker. Nucleus is stained with NucBlue (blue).