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Neuronal differentiation. (A) Day 7 of neural induction, the iPSC morphology is lost and the culture is ready to be harvested and expanded. (B) Passage 4 NSC culture. (C) At day 4 of terminal neuronal differentiation the cells begin to form a neuronal network. (D) Tubulin beta 3 class III (green) staining of neuronal cells at day 24 of terminal neuronal differentiation. Counter staining of the nucleus was conducted by DAPI staining (blue).
