Table 1.

Comparison of current protocol with previous published keratinocyte (KER) reprogramming protocols.

Publication	KER source	KER isolation method	KER growth on feeder-layer (yes/no)	KER cultured in serum containing medium	Reprograming delivery method (yes/no)	Serum containing medium during reprogramming	Reprogramming on feeder-layer (yes/no)	Addition of ROCK inhibitor for reprogramming (yes/no)
Current method	Plucked hairs	Trypsinization	No	No	Sendai Virus	No	No	Yes
Aasen et al., 2008	Foreskin/plucked hair	Follicle outgrowth	No	Yes	Retrovirus	No	Yes	No
Aasen and Belmonte, 2010	Foreskin/skin biopsies/plucked hair	Trypsinization/follicle outgrowth	No	No	Retrovirus	No	Yes	No
Petit et al., 2012	Plucked hair	Trypsinization	Yes	Yes	Lentivirus	No	Yes	No
Mandel et al., 2012	Plucked hair	Trypsinization	Yes	Yes	Lentivirus (excitable)	Yes	Yes	No
Ohmine et al., 2012	Skin biopsy	Enzymatic	No	No	Lentivirus	No	N/a	No
Linta et al., 2013	Plucked hair	Follicle outgrowth	No	No	Lentivirus	No	Yes	No
Umegaki-Arao et al., 2014	Skin biopsy	Enzymatic	N/A	N/A	Retrovirus	No	Yes	No
Piao et al., 2014	Epidermal	Commercial line	No	N/A	Episomal	No	Yes	No
Hung et al., 2015	Plucked hair	Follicle outgrowth	No	No	Episomal	No	Yes	No
Lim et al., 2016	Epidermal	Commercial line	N/A	No	Retrovirus	N/A	Yes	No
Matsumura et al., 2018	Skin biopsy	Enzymatic	Yes	No	Sendai virus	No	Yes	No
Nakayama et al., 2018	Skin biopsy	Enzymatic	Yes	No	Sendai virus	No	Yes	No
Boonkaew et al., 2018	Plucked hair	outgrowth	No	Yes	Sendai virus	No	No	No

KER, Keratinocyte; N/A, not available.