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. 2018 Sep 25;9:2250. doi: 10.3389/fmicb.2018.02250

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Copyright © 2018 Chen, Zhang, Lin, Li, Lan, Song, Gao, Wang, Xie, Li and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The 3′ UTR is essential for viral genome replication. (A) 0.8 μg of the in vitro transcribed products of pR-DHAV-1 or pR-DHAV-Δ3′UTR-A₂₅ were transfected into DEFs, cell lysates were collected from 12 to 60 hpt and viral copy number was measured by RT-qPCR. (B) Equal amount of in vitro transcribed product of pR-DHAV-1 or pR-DHAV-R3′UTR-A₂₅ were transfected into DEFs, cell lysates were collected at 4 hpt to measure the remaining amount of viral RNAs. (C) Equal amount of 0.8 μg of the in vitro transcribed products of pR-DHAV-1 or pR-DHAV-R3′UTR-A₂₅ were transfected into DEFs to measure viral growth characteristics as previously described. Bars represent the means ± standard deviations of three replicate experiments.