FIGURE 5.

The effect of the DHAV-1 3′ UTR plus the poly(A)₂₅ tail on IRES-mediated translation efficiency. 0.8 μg of of the in vitro transcribed products of the linearized plasmids pDHAV-3′UTR-A₂₅ and pDHAV-Δ3′UTR-ΔA₂₅ were co-transfected into DEFs with 0.1 μg of pGMLR-TK plasmid, and the FLuc/RLuc activities were measured from 4 to 12 hpt. Columns and bars represent the means and standard deviations, respectively, of three independent transfections. ^∗P < 0.05; ^∗∗∗P < 0.001.