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. 2018 Oct;59(10):1531–1537. doi: 10.2967/jnumed.117.206714

FIGURE 1.

FIGURE 1.

89Zr-oxine does not affect cell viability or function at optimized cell labeling activity. After 89Zr-oxine labeling at specified specific activities, IL13Rα2-CAR T cells were cultured under standard conditions for up to 144 h. (A) Viability of untransduced (mock) and IL13Rα2-CAR T cells was monitored over 144 h of cell culture. (B) IL13Rα2-CAR T cell killing of PBT030-2 ffLuc-positive cells was assessed in 7-h luciferase cytotoxicity assay at 10:1 T cell–to–tumor ratio (n = 6) at specified time points after 89Zr-oxine labeling and subsequent cell culturing. (C) Enzyme-linked immunosorbent assay for IFN-γ cytokine production of 89Zr-oxine–labeled IL13Rα2-CAR T cells after incubation with plate-bound IL13Rα2 for 4 h (n = 2). (D) IFN-γ production determined by cytokine bead analysis of 89Zr-oxine–labeled IL13Rα2-CAR T cells after overnight incubation with PBT030-2 ffLuc-positive cells. (E) Migration of IL13Rα2-CAR T cells to U251T conditioned medium or control medium in 4-h Boyden chamber chemotaxis assay (n = 4). All data are expressed as mean ± SE.