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Real-time RT-PCR. Induction of cytokines in Raw 264.7 macrophages by recombinant GALT stimulation. Raw 264.7 macrophages were incubated with 10 μg/mL GALT and 200 ng/mL LPS (positive control) for 12 h, as well as single culture media (negative control). Quantification analysis was conducted to analyze the transcriptional levels of TNF-α, IL-1β, IL-6. β-actin was chosen to be a reference gene in relatively quantification analysis.
