Table 1.

Computational tools available for designing guide RNAs.

Name	Homepage	Available RGN	Off-target scoring	Predicts gRNA activity	Off-target hits	References
Cas-OFFinder	http://www.rgenome.net/cas-offinder/	7	no	no	yes	[44]
Benchling	https://benchling.com/	1	yes	yes	yes
Deskgen	https://www.deskgen.com/landing/	1	yes	yes	yes
CRISPR Design	http://crispr.mit.edu/	1	yes	no	yes	[45]
E-CRISPR	http://www.e-crisp.org/E-CRISP/	1	yes	yes	yes	[46]
CHOPCHOP	http://chopchop.cbu.uib.no/	2	no	yes	yes	[47, 48]
ZiFiT	http://zifit.partners.org/ZiFiT/CSquare9Nuclease.aspx	1	yes	no	yes	[49]
sgRNA Designer	https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design	2	no	yes	yes	[50, 51]
CRISPRseek	http://bioconductor.org/packages/release/bioc/html/CRISPRseek.html	1	yes	yes	no	[52]
CRISPR-Era	http://crispr-era.stanford.edu/	1	yes	yes	no	[53]

∗Supplementary file: workflow to design in silico gRNAs.