Table 2.

Delivery of CRISPR/Cas9 components in different formats.

	Plasmid DNA	Cas9 mRNA + sgRNA	Cas9 Protein + sgRNA
Efficiency	++++	++	++
Specificity	++	++++	++++
Cell mortality	++++	+	+
Off-targets	++++	+	+
Stress to cells	++++	+	+
Onset of action	+	++	++++
Half life	++++	++	+
Risks of random integration	++++	#	#

#, no risk of integration.