Figure 3.
Effects of sbm Downregulation in Glia on Various Tissue Growth
(A) Fat body cell area (μm2) through time and representative pictures of fat bodies stained with Sudan black to show fat accumulation (the red broken line exemplifies some measured cells in each picture) (two-way ANOVA and Duncan post-test, p < 0.01, F = 6.26; n = 4–5.)
(B) Wing imaginal disc area (mm2) through time and representative pictures (Two-Way ANOVA with a Duncan Post-Test, interaction between the variables time and genotype is not significant so only statistical differences between time points is shown in the graph, p < 0.0001, F = 123.13; n = 11–15).
(C) Estimation of larval single brain lobe volume (mm3) through time and representative pictures (two-way ANOVA with a Tukey post-test, p < 0.0001, F = 22.55; DMS = 0.34; n = 4–5.)
In all statistical analyses, data were transformed to natural logarithm (ln) to fulfill the test criteria. In all graphs, points with different letters signify that they are significantly different (p < 0.05). Data represent mean ± SEM; the percentages above points indicate the difference in respect to the control. hAEL, hours after egg laying.