Skip to main content
. 2018 Sep 18;24(12):3194–3206.e4. doi: 10.1016/j.celrep.2018.08.059

Figure 3.

Figure 3

Scaf Is Expressed in the Subesophageal Zone of the Brain and the Ventral Nerve Cord

(A–B′′) The SEZ of adult brains from R50H04-GAL4; UAS-6XGFP flies double stained using anti-GFP (green) and anti-Scaf (magenta) antibodies demonstrating that Scaf is expressed in R50H04 neurons. Arrowheads mark the location of cell bodies expressing Scaf and GFP. (A)–(A′′) and (B)–(B′′) represent anterior and posterior focal planes, respectively.

(C) Gene structure of scaf. Black rectangles indicate UTRs, and white rectangles indicate coding exons. The blue bars indicate the insertion site of the protein trap and the intronic region used to generate the R50H04-GAL4 line.

(D and E) Adult fly brain (D) and VNC (E) from R50H04-GAL4; UAS-mCD8::GFP flies stained with an anti-GFP antibody (white) showing the cell bodies and neuronal processes of Scaf neurons.

(F and G) Schematic representation of the cell bodies and projections of Scaf neurons in brain (F) and VNC (G).

Scale bars, 50 μm. See also Figures S3–S5 and Videos S1 and S2.