Figure 6.

Scaf and Scaf Neurons Promote Feeding on Nutritious Sugar Independent of the State of the Fly

(A) Overexpression of scaf does not result in enhanced PER at varying concentrations of sucrose (n = 4).

(B) TrpA1-mediated activation of Scaf neurons does not lead to increased PER in sated flies at varying concentrations of sucrose (n = 4). Scaf neurons were activated by expressing dTrpA1 using R50H04-GAL4 by shifting the flies to 31°C for 1 hr.

(C and D) Activation of Scaf neurons does not alter feeding preference. Scaf neurons were activated by expressing dTrpA1 using R50H04-GAL4 in sated flies. Preference was tested at 31°C under darkness for 2 hr. ^∗p ≤ 0.05, ^∗∗∗∗p ≤ 0.0001.

(C) Activation of Scaf neurons does not alter the feeding preference when given a choice between sucrose and water (n = 4).

(D) Enhanced feeding caused by activation of Scaf neurons is directed toward nutritious sugars when given a choice between D- and L-glucose (n = 3–4).

(E and F) Overexpression of scaf does not alter the feeding preference in flies. Scaf was overexpressed using R50H04-GAL4 in sated flies. Preference was tested at 25°C under darkness for 2 hr.

(E) Overexpression of Scaf does not alter the preference of feeding when given a choice between sucrose and water (n = 3–4).

(F) Enhanced feeding caused by overexpression of scaf is directed toward nutritious sugars when given a choice between D- and L-glucose (n = 5–6).

Bar graphs and line graphs represent the mean. Error bars represent SEM.