Figure 4.

Maturation in S. cerevisiae depends on the expression method and affects FRET, as determined by wide-field fluorescence microscopy. The FRET values are lower than in, e.g., in Figure 2, because those were measured on a confocal microscope. (A) Comparison of the FRET/mCerulean3 ratios of the different expression conditions from data presented in Figure S3. (B) mCitrine/mCerulean3 ratios show that maturation affects the accuracy of the crowding read-out.