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. 2018 Oct 2;5:7. doi: 10.1186/s40661-018-0064-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — PNC prevalence in ovarian cancer cell lines. a Immunofluorescent staining was done in SKOV3 and OVCAR3 for PTB, a component of PNC (green, marked by arrows) that are located adjacent to nucleoli (fibrillarin staining in red) within the nucleus (DAPI staining in blue). Bar = 5 μm. b and c SKOV3 and d and e OVCAR3 cells were treated with ML246 at 0.05uM, 1uM or 2 uM or DMSO for 24 h and % PNC prevalence as well as cells carrying multiple PNCs was calculated. * p < 0.05