Table 1.
Blood cancer cell lines carry nonproductive BCR/TCR in either both alleles or the only expressed allele
| Cell lines | Reads | Fraction % | V genes | D genes | J genes | CDR3 Sequences | |
|---|---|---|---|---|---|---|---|
| B-ALL | NALM-19 | 425 | 36.08 | IGHV1-3 | IGHD6-6 | IGHJ4 | CARDRV*QL_PPPLRDYW |
| MHH-CALL-4 | 214 | 14.33 | TRAV8-2 | TRAJ8 | CVVGL_QKLVF | ||
| 163 | 10.92 | IGHV2-26 | IGHD2-21 | IGHJ5 | CARIRPRAVR_CGSP*GPDPW | ||
| KASUMI-2 | 3491 | 60.82 | IGLV1-40 | IGLJ3 | CQSYD_AGVF | ||
| MUTZ-5 | 1632 | 76.30 | IGLV2-34 | IGLJ2 | CSSYA_HLVVF | ||
| REH | 100 | 16.21 | IGHV3-15 | IGHD3-10 | IGHJ6 | CTTGMVRGVI_YYYYGMDVW | |
| RS4;11 | 361 | 26.05 | IGHV6-1 | IGHD1-20 | IGHJ4 | CAREP*LELFDYW | |
| 241 | 17.39 | IGHV3-20 | IGHD2-8 | IGHJ5 | CARD*SRY*W_VCYTDWFDPW | ||
| 173 | 12.48 | IGLV11-55 | IGLJ7 | CAMG_PQF | |||
| 36 | 2.60 | IGLV4-3 | IGLJ3 | CGESHTIDG_SRLRFWVF | |||
| NALM-6 | 15670 | 74.70 | IGLV2-14 | IGLJ7 | CSSYTSS_ALGAVF | ||
| 2479 | 11.82 | IGLV4-3 | IGLJ3 | CGESHTIDGQ_RLQAPGGVF | |||
| 2229 | 10.63 | IGHV1-69 | IGHD3-10 | IGHJ6 | CARDRRGEWPPSDYYYYYMDVW | ||
| T-ALL | KE-37 | 656 | 56.21 | TRBV3-2 | TRBD1 | TRBJ1-5 | CASSQDSGTG_RVGNQPQHF |
| ALL-SIL | 2364 | 51.42 | TRGV7 | TRGJ1 | CATWGSG_YYKKLF | ||
| 909 | 19.77 | TRBV18 | TRBD1 | TRBJ2-1 | CASSPMEK_GHKGEQFF | ||
| 482 | 10.49 | TRBV7-9 | TRBD1 | TRBJ2-7 | CASSLDT_WYEQYF | ||
| 221 | 4.81 | TRGV9 | TRGJ1 | CALWR*_YYKKLF | |||
| 86 | 1.87 | TRDV2 | TRDJ1 | CACDK_DKLIF | |||
| MOLT-3 | 2913 | 6.95 | TRBV10-3 | TRBJ2-5 | CAISEPTG_SEETQYF | ||
| 231 | 0.55 | TRAV1-1 | TRAJ33 | CAVRDHPW_SNYQLIW | |||
| 97 | 0.23 | TRAV1-1 | TRAJ24 | CAVKMEQ_WGKLQF | |||
| B cell lymphoma unspecified | JM1 | 10946 | 48.32 | IGLV3-1 | IGLJ6 | CQAWD_QPNVF | |
| 7877 | 34.78 | IGLV3-10 | IGLJ6 | CYSTDSS_VIIANVF | |||
| 1135 | 5.01 | IGLV8-61 | IGLJ7 | CV_VF | |||
| HT | 18074 | 89.54 | IGKV3-11 | IGKJ5 | CQQRTNWPITF | ||
| 1488 | 7.37 | IGHV3-53 | IGHD1-1 | IGHJ4 | CARASFAT_*LYFDSW | ||
| Blast phase CML B-ALL# |
BV-173 | 764 | 57.83 | IGHV3-21 | IGHD2-15 | IGHJ3 | CASQIL*WW*_PYRGAFDIW |
| 157 | 11.88 | IGKV2-29 | IGKJ3 | *MQGIH_SSLFTF | |||
| 35 | 2.65 | TRAV8-7 | TRAJ19 | CAGADRLQTGMRGAF | |||
| DLBCL | Toledo | 11815 | 58.54 | IGLV2-14 | IGLJ7 | CSSYTS_QHSVF | |
| 6830 | 33.84 | IGLV3-21 | IGLJ6 | CQVWDSS_*SPNVF | |||
| 316 | 1.57 | IGLV8-61 | IGLJ7 | CV_VF | |||
Asterisks “*” indicate stop codon; underscores “_” indicate out of frame CDR3 translation
# BV-173 is also been regarded as an B cell precursor ALL cell lines [47] despite its original establishment from a patient with blast phase CML [48] (https://www.dsmz.de/catalogues/details/culture/ACC-20.html)