Fig. 9.

Ibuprofen treatment of non-tumor baring involution hosts has no observed impact on reproductive success or immune reactivity. a Numbers of pups born and successfully nursed for 10 days (start of synchronized mammary gland involution) at each cycle of breeding with (+) or without (−) 10 days of ibuprofen (IBU) after each round of weaning-induced involution (n = 10–15 mice/group). b Assessment of anti-dsDNA antibodies in the circulating maternal blood after completion of the third pregnancy/lactation/involution cycle. Maximum linear range of anti-ds-DNA detection is 1000 IgG U/mL, and gray dashed line indicates level of anti-dsDNA antibodies commonly observed in spontaneous autoimmune mouse serum. c Representative H&E sections from mice after completion of the third pregnancy/lactation/involution cycle (Multi-parity) in the presence or absence of 10 days of ibuprofen (IBU) after each round of weaning-induced involution. d Assessment of antigen specific naïve T cell activation. Assay was established as outlined in Additional file 4: Figure S4. Antigen specific T cell activation is expressed by fold change in absolute number of antigen specific Do11.10 T cells in the draining lymph node of ova injected to PBS control in nulliparous (NP) or involution (INV) mice with or without ibuprofen (IBU). Determination of statistical differences between groups was based upon one-tailed Student’s t-test according to previously published results with this assay, p < 0.05 (*). e graphical summary reported results