Table 5.
Libraries prepared from human gDNA by manual preparation and two automated liquid handler platforms
| Metrics | Manual Preparation | Hamilton NGS STAR™ | Eppendorf epMotion® 5075 t | |
|---|---|---|---|---|
| Yield (Qubit), ng/μl | Run 1 Run 2 |
12.4–14.1 11.1–13.5 |
9.1–11.1 13.7–15.6 |
12.8–14.2 14.1–17.4 |
| Yield CV, % | Run 1 Run 2 |
5.1 6.2 |
8.3 4.8 |
3.5 7.3 |
| Index CV, % | Run 1 Run 2 |
10.8 12.1 |
13.7 11.7 |
11.0 12.6 |
| Median insert size, bp (optimal: 350 ± 50 bp) |
Run 1 Run 2 |
348–357 350–363 |
375–391 377–385 |
335–344 351–368 |
| Autosome mean coverage, X | 30–32 | 30–32 | 30–32 | |
| Coverage across the human genome at 15X, % | 97.6 | 97.7 | 97.4 | |
| Coverage across exonic regions at 10X, % | 99.6 | 99.6 | 99.8 | |
| Mean diversity | > 2.0e9 | > 2.0e9 | > 2.0e9 | |
| Autosome Callability, % | 95.0 | 94.5 | 94.5 |
Libraries were prepared from 200 to 300 ng of human DNA (NA12878) using an Illumina Qualified method. Data is presented for two 8-plex runs on a HiSeqX system, with sequencing reads trimmed to a 30X depth (380 million reads, 2 × 151 bp). Data analysis performed using the BaseSpace Sequence Hub Whole Genome Sequencing v5.0 App. CV Coefficient of variation