Skip to main content
. 2018 Sep 25;9:1076. doi: 10.3389/fphar.2018.01076

FIGURE 5.

FIGURE 5

Budlein A reduces in vivo and in vitro MSU-induced IL-1β and TNF-α production. Fifteen hours after the injection of MSU crystals, knee joint was dissected for the determination of Tnf-α (A) and Il-1β (B) mRNA expression by RT-qPCR. Results are expressed as mean ± SEM, n = 6 mice per group per experiment, two independent experiments. ∗p < 0.05 vs. saline group; #p < 0.05 vs. 0 mg/kg group, one-way ANOVA followed by Tukey’s post hoc. For the in vitro assay, BMDMs were pre-treated with budlein A at 1, 3, or 10 μg/mL 30 min before stimulus with LPS for the determination of TNF-α levels (C) by ELISA. For the determination of IL-1β levels (D) by ELISA, LPS-primed BMDMs were treated with budlein A at 1, 3, or 10 μg/mL 30 min after stimulus LPS. Supernatants were collected 5 h after MSU (C,D). ∗p < 0.05 vs. saline group; #p < 0.05 vs. 0 mg/kg group, one-way ANOVA followed by Tukey’s post hoc.