Effects of WD and VC on cardiac lipid accumulation and oxidative stress. (A) Cardiac lipid accumulation was evaluated by ORO staining (400×, scale bar = 50 μm) and (B) quantification of ORO staining for lipid accumulation. Data were presented as means ± SD. Protein expression of(C) 4-HNE, (D) 3-NT, (E) CAT, NQO-1, and HO-1, and (F) MT were determined by Western blot. Data were presented as means ± SD.