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. 2018 Sep 25;9:2224. doi: 10.3389/fmicb.2018.02224

FIGURE 3.

FIGURE 3

Detection limits of IS6110 and senX3 PCR assays. Curves determined by probit analysis (95% probability detection). (A) IS6110 and senX3 PCR using M. tuberculosis mc27000 DNA. LOD were estimated at 2.03 log10 (107 copies/ml) and 2.89 log10 (741 copies/ml), respectively. (B) The detection limit of M. bovis BCG was 2.40 log10 and 2.90 log10 for IS6110 and senX3 PCR assays, respectively. (C) IS6110 PCR detection limits using two or three sets of primers compared to a single set of primers based on M. tuberculosis mc27000 strain were estimated at 2.1, 2.2, 2.3, 2.35, 2.4 log10, respectively for ISP, Triplex, ISP+ISM, ISP+ISL, and ISM+ISL.