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. 2018 Sep 25;9:2224. doi: 10.3389/fmicb.2018.02224

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Copyright © 2018 Kolia-Diafouka, Godreuil, Bourdin, Carrère-Kremer, Kremer, Van de Perre and Tuaillon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of DNA extraction protocols in spiked sputum samples. The M. tuberculosis mc²7000 stock suspension was diluted and used to spike negative sputum samples. Box plots with C_T median, 10th, 25th, 75th, and 90th centiles of 10 replicates. Methods are indicated by colors: brown: Chelex^® method; pink: Guanidium Isothicyanate/Tris-HCl/EDTA + 3 cycles of freeze thawing and boiling; black: Tween 20/Tris-HCl/EDTA/lysozyme+proteinase K/SDS + warming cycles 56°C/95°C; green: Nonidet P-40/Tris-HCl/EDTA/lysozyme+proteinase K/SDS + warming cycles 56°C/95°C; blue: Triton X-100/Tris-HCl/EDTA; purple: NaOH + boiling and sonication.