Table 2.

Intra run and inter run variability.

	In-house qPCR IS6110			In-house qPCRsenX3
	Mean (Log10 fg/μl)	SD	%CV	Mean	SD	%CV
INTRA RUN MTB
6 log 10	5.85	0.05	1.36	5.53	0.17	0.70
5 log10	5.07	0.09	2.03	4.73	0.11	0.45
4 log10	3.74	0.19	3.55	3.17	0.07	0.34
3 log10	2.53	0.16	2.27	2.59	0.13	2.29
2 log10	1.70	0.08	1.14	ND	ND	ND
1 log10	0.80	0.11	6.25	ND	ND	ND
INTRA RUN BCG
6 log 10	5.55	0.52	3.26	5.70	0.51	2.61
5 log10	4.67	0.05	3.16	4.22	0.15	0.66
4 log10	3.40	0.22	2.56	3.15	0.41	1.41
3 log10	2.73	1.19	5.31	2.56	0.25	0.76
2 log10	1.86	0.10	2.53	1.53	0.82	2.27
1 log10	0.83	0.66	18.32	ND	ND	ND
STANDARD
5 log 10	4.99	0.12	0.46	4.89	0.05	4.14
4 log10	3.98	0.27	0.88	3.88	0.07	0.61
3 log10	3.07	0.10	0.30	3.00	0.00	0.84
2 log10	1.96	0.83	2.21	ND	ND	ND
Average Mtb	1.86	0.11	2.76	4.01	0.12	0.95
Average BCG	0.83	0.45	3.02	3.43	0.43	1.54
INTER RUN
Operator 1
5 log10	4.96	0.04	4.00	4.95	0.15	3.16
4 log10	3.99	0.14	0.60	4.01	0.19	4.60
3 log10	3.02	0.05	0.55	2.99	0.25	2.55
2 log10	1.94	0.09	6.23	1.98	0.11	2.70
Operator 2
5 log10	4.97	0.02	6.15	4.93	0.05	3.15
4 log10	4.01	0.03	4.80	4.07	0.19	4.60
3 log10	2.99	0.44	1.55	2.96	0.64	2.55
2 log10	2.10	0.17	15.35	1.97	0.20	3.53
Average	3.52	0.17	6.96	3.98	0.25	2.96