FIGURE 5.

(A) Germination of SR7 spores under 1 atm CO₂ in LB medium, 100 mM L-alanine-amended LB, or LB heated at 65°C for 15 min. Open triangles represent viable spore counts based on heat-killed (80°C for 15 min) CFU counts. (B) Syto9 cell staining patterns of SR7 spores and vegetative cells. ‘Edge,’ ‘Partial,’ and ‘Complete’ refer to Syto9 stain localization/distribution within the cell. (C) Extent of population-wide germination in PBS incubations amended with L-alanine, L-leucine or heat activated, as measured by percent of population displaying dormant or germinated cell staining patterns assessed by microscopy. (D) Bulk fluorescence of samples with different germination treatments.