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. 2018 Sep 25;9:2152. doi: 10.3389/fmicb.2018.02152

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Copyright © 2018 Freedman, Peet, Boock, Penn, Prather and Thompson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Sequence-based comparison of the largest B. megaterium SR7 contig to reference genome B. megaterium QM B1551 (B) ANI-based comparison showing synteny between SR7 and B. megaterium QM B1551 and B. aryabhattai GZ03 (C) Schematic of the B. megaterium SR7 5.51 Mbp genome, including the closed 5.45 Mbp chromosome. Concentric circles (outside in) are RAST ORFs (blue), rRNA and tRNA (green lines on gray circle), GC content, and GC skew. Asymmetry in GC skew indicates proper chromosome assembly (Grigoriev, 1998). Circles at right represent five putative plasmids (p1–p5) native to SR7. (D) Venn diagram displaying shared RAST-called and annotated ORFs between SR7 and three previously sequenced B. megaterium strains. 366 RAST-called ORF annotations unique to SR7 are listed in Supplementary Table S7.