Table 1.
Summary of scCO2-resistant strain isolation results.
| (A) McElmo Dome fluid-inoculated mixed community enrichment culture and passaging | ||||
| Passage | Duration | Well | Media | Max cells/ml |
| M1 | 45 days | 2 | MS-SR | 5.4 × 107 |
| 4 | MS-SR | 2.3 × 107 | ||
| 7 | MS-SR | 3.0 × 106 | ||
| M2 | 19 days | 2 | MS-SR | 1.6 × 105 |
| 4 | MS-SR | 1.9 × 104 | ||
| 7 | MS-SR | 2.1 × 107 | ||
| M3 | 33 days | 2 | MS-SR | 4.6 × 104 |
| 4 | MS-SR | b.d. | ||
| 7 | MS-SR | 6.0 × 106 | ||
| M4 | 35 days | 7 | MS-SR | 6.9 × 106 |
| 7 | LB | 7.8 × 106 | ||
| (B) Isolate SR7 pure culture scCO2 incubations | ||||
| Incubation | Duration | Media | Growth | Max cells/ml |
| P1 | 33 days | MS-SR | 1/2 | 1.0 × 108 |
| P2 | 28 days | MS-SR | 1/3 | 2.0 × 107 |
| LB | 3/3 | 6.8 × 107 | ||
| P3 | 42 days | LB | 3/3 | 3.5 × 107 |