Figure 2.

Overexpression of miR-1225-5p suppresses the proliferation of glioblastoma cells in vitro and in vivo.

Notes: (A) The expression of miR-1225-5p in U87 and U87-1225 (stably expressing miR-1225-5p) cells was measured by reverse transcription PCR (RT-PCR) and normalized against U6. (B) U87 and U87-1225 cells were subjected to an MTT assay to assess cell proliferation at each time point. (C) Representative image of crystal violet-stained cell colonies analyzed by colony-formation assay at 14 days. (D) Quantification plots of (C). (E) Tumor-growth curves demonstrated that miR-1225-5p inhibited xenograft tumor growth in nude mice. (F) Relative tumor weight at 22 days after inoculation. **P<0.01 or ***P<0.001 vs U87 group.