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. 2018 Sep 28;11:6305–6316. doi: 10.2147/OTT.S172152

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© 2018 Han et al. This work is published and licensed by Dove Medical Press Limited

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Effects of miR-218 overexpression on tumorigenesis of PTC in vitro.

Notes: K-1 and TPC-1 cells were transfected with miR-218 or miR-control, and further experiments were performed at 24 or 48 hours post-transfection. (A) qRT-PCR analysis of miR-218 expression in transfected K-1 and TPC-1 cells. (B) CCK-8 assay was conducted in transfected K-1 and TPC-1 cells to measure the cell viability. Cell invasive ability was examined by Transwell invasion assay in transfected (C) K-1 and (D) TPC-1 cells (magnification ×200). Flow cytometry analysis of apoptosis in transfected (E) K-1 and (F) TPC-1 cells. *P<0.05.

Abbreviations: CCK-8, cell counting kit-8; FITC, fluorescein isothiocyanate; PI, propidium iodide; PTC, papillary thyroid cancer; qRT-PCR, quantitative real-time PCR.

Effects of miR-218 overexpression on tumorigenesis of PTC in vitro.

Notes: K-1 and TPC-1 cells were transfected with miR-218 or miR-control, and further experiments were performed at 24 or 48 hours post-transfection. (A) qRT-PCR analysis of miR-218 expression in transfected K-1 and TPC-1 cells. (B) CCK-8 assay was conducted in transfected K-1 and TPC-1 cells to measure the cell viability. Cell invasive ability was examined by Transwell invasion assay in transfected (C) K-1 and (D) TPC-1 cells (magnification ×200). Flow cytometry analysis of apoptosis in transfected (E) K-1 and (F) TPC-1 cells. *P<0.05.

Abbreviations: CCK-8, cell counting kit-8; FITC, fluorescein isothiocyanate; PI, propidium iodide; PTC, papillary thyroid cancer; qRT-PCR, quantitative real-time PCR.