Figure 7.

miR-218 overexpression inhibits PTC tumor growth in vivo.

Notes: TPC-1 cells transfected with lentiviral vectors containing miR-218 or miR-control were injected into nude mice. The mice (A) body weights and (B) tumor volumes were monitored every 5 days. (C) The mice were killed after 35 days, and the tumor weights were measured. The expression of Runx2 at mRNA and protein levels in removed tumors was assessed by (D) qRT-PCR and (E) Western blot. (F) The protein levels of phosphorylated AKT (p-AKT) (Ser473), PTEN, and total AKT (t-AKT) in excised tumors were determined by Western blot. (G) The protein levels of p-mTOR, t-mTOR, p-GSK-3β, and t-GSK-3β in excised tumors were examined by Western blot. *P<0.05.

Abbreviations: PTC, papillary thyroid cancer; qRT-PCR, quantitative real-time PCR.