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. Author manuscript; available in PMC: 2018 Oct 2.
Published in final edited form as: J Am Chem Soc. 2013 Oct 16;135(43):16018–16021. doi: 10.1021/ja408093p

Figure 2. Compound 7 stains macrophages undergoing phagosomal acidification.

Figure 2

RAW264.7 cells (preincubated or not with zymosan) were treated with 7 (100 nM) for 15 min and imaged by confocal microscopy. Fluorescence staining of 7 in (a) nonactivated macrophages, (b) zymosan-activated macrophages, and (c) zymosan-activated macrophages treated with bafilomycin A (100 nM); (d) quantification of fluorescence emission represented as means (n = 4) and error bars as SD, *** for p < 0.005 compared to b. Scale bar: 20 μm.