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. Author manuscript; available in PMC: 2018 Oct 2.
Published in final edited form as: J Am Chem Soc. 2013 Oct 16;135(43):16018–16021. doi: 10.1021/ja408093p

Figure 4. PhagoGreen enables in vivo imaging of phagocytic macrophages.

Figure 4

Transgenic zebrafish with mCherry-labeled macrophages were treated with PhagoGreen (600 nM) for 30 min and imaged by confocal microscopy. (a) Images from a time-lapse movie (Video S1) showing the phagosomal localization of PhagoGreen (white arrowheads) demonstrated by the surrounding red fluorescence of cytoplasmic mCherry; (b) single channel image of (a) showing brighter fluorescence in mature phagosomes (yellow arrowheads); (c) i–iv: sequential images from a time-lapse movie (Video S2) of an actively engulfing macrophage, where the mature phagosome is brightly green fluorescent while the newly formed phagosome is devoid of green fluorescence (yellow arrows). Scale bars (a,b): 20 μm, (c): 15 μm.