Table 2. P-values obtained for the double generalized linear model.
ddPCR | Bioanalyzer | Densitometry_ImageJ | Densitometry_GeneTools | |||||
---|---|---|---|---|---|---|---|---|
mean | dispersion | mean | dispersion | mean | dispersion | mean | dispersion | |
Lab 1 | - | 1.89E-11 | 3.06E-01 | - | - | |||
Lab 3 | 6.85E-03 | 8.16E-01 | 1.32E-48 | 2.34E-09 | 1.61E-03 | 5.96E-48 | - | |
Lab 4 | - | 3.53E-07 | 2.45E-13 | 2.02E-07 | 1.89E-32 | 1.32E-01 | 1.90E-27 | |
Lab 5 | - | 8.37E-40 | 9.34E-01 | 5.37E-04 | 3.25E-25 | - | ||
Lab 6 | - | 7.15E-95 | 1.41E-06 | 1.89E-13 | 5.82E-25 | - |
P-values obtained for the double generalized linear model for the mean and the dispersion considering different AON concentrations and sample sets. The ddPCR performed by lab 2 was used as reference. P-values less than 0.05 were considered significant. Significant P-values indicate a less reliable quantification method due to a higher variation to the reference, not significant P-values indicate a reliable method and are shown in bold.