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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Virol Methods. 2018 Aug 9;261:71–79. doi: 10.1016/j.jviromet.2018.08.004

Table D.1.

Detection of CHIKV RNA in infected Ae. aegypti mosquitoes on Q-paper. Fluorescent signal: (+), low, <1000 median fluorescence intensity (MFI); (+++), high >5,000 MFI; (−) not detectable. A note: lower viral titers are expected in the legs than in the bodies, as shown previously for DENV and CHIKV infection (Richards et al. 2010, Alto and Bettinardi 2013).

Q-paper Identity, # (Table1) CHIKV titers in mosquito legs by real time-PCR, genome equivalents/mL Q-paper’s texture CHIKV RNA detection by Luminex DHA
Water-wash Eluted by 1M NaCl solution
1 6.86 ×104 brittle +
2 9.14 ×104 brittle
3 1.2 ×105 brittle
4 1.63 ×104 brittle +
5 3.72 ×104 pliable +++ +++
6 3.17 ×104 pliable +++ +++
7 5.79 ×104 pliable ++ +++
8 6.86 ×104 pliable +++
9 9.14 ×104 brittle
10 1.2 ×105 brittle +
11 1.63 ×104 brittle +++ +
12 3.72 ×104 brittle
13 3.17 ×104 pliable +++ +++
14 5.79 ×104 pliable +++ +++
15 6.86 ×104 pliable +++
16 9.14 ×104 pliable ++ +++