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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Virol Methods. 2018 Aug 9;261:71–79. doi: 10.1016/j.jviromet.2018.08.004

Table 2.

Standard and self-avoiding molecular recognition system (SAMRS) oligonucleotides used for detection of ZIKV and CHIKV RNA. All reverse primers are 5’-biotinylated. All probes were 5’-amino-C12-modified. P, artificially expanded genetic information system (AEGIS) nucleotide; *, SAMRS nucleotides.

Oligonucleotides Forward (F) and Reverse (R) primers’ sequences Luminex probe sequence
CHIKV, standard nucleotides F_CHIKV:
CAGATGGCAACGAACAGG
R_CHIKV:
GGGTCCTCTGAGCTTCTC		 CCTTTGCAAGCTCCAGATC
CHIKV, SAMRS nucleotides F_CHIKV:
CAGATGGCAACGAA*C*A*G*G
R_CHIKV:
GGGTCCTCTGAGCT*T*C*T*C 		CCTTTPCAAPCTCCAPATC
ZIKV, standard nucleotides F_ ZIKV:
AGGGACCTCCGACTGATG
R_ ZIKV:
CCTCAATCCACACTCTGTTC		 GAAAGGGAGAATGGATGACC
ZIKV, SAMRS nucleotides F_ ZIKV:
AGGGACCTCCGACT*G*A*T*G
R_ ZIKV:
CCTCAATCCACACTCT*G*T*T*C 		PAAAPPPAPAATPPATPACC