Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 1;150(10):1360–1372. doi: 10.1085/jgp.201711979

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Burger et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 9. — Positive allosteric modulation of monomeric M₄ mAChRs. (A and B) [³H]NMS interaction binding studies between the PAM, LY2033298, and acetylcholine at the M₄ mAChR expressed in either CHO cells (A; replotted from Leach et al., 2010) or purified from Sf9 cells (B; M₄R-mT4L; for methods, see Thal et al., 2016) and reconstituted into rHDL particles, as was previously done for rhodopsin (Whorton et al., 2007). Nearly identical levels of cooperativity were observed, indicating that LY2033298 is able to influence the binding of acetylcholine in a monomeric mAChR and is not dependent on oligomerization status of the receptor. Data from B represent the mean ± SEM of n = 3 experiments performed in duplicate.